NAD⁺ Phosphate Buffered Solution Overview
NAD⁺ Phosphate Buffered Solution is a compounded aqueous research formulation containing nicotinamide adenine dinucleotide (NAD⁺) at a concentration of 50 mg/mL, prepared in a phosphate-buffered system to enhance pH stability and molecular integrity. The formulation includes 1% benzyl alcohol as a preservative.
In laboratory research, phosphate-buffered NAD⁺ solutions are commonly used in studies involving cellular redox balance, mitochondrial metabolism, enzymatic cofactor activity, and NAD⁺-dependent signaling pathways.
This formulation is not approved for human or veterinary use and is supplied strictly for laboratory and analytical research purposes.
Mechanism of Action
NAD⁺ functions as a central redox coenzyme in cellular metabolism. In research models, it is essential for:
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Electron transfer reactions in oxidative phosphorylation
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Regulation of NAD⁺/NADH redox balance
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Activity of sirtuins, PARPs, and other NAD⁺-dependent enzymes
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Mitochondrial and cytosolic metabolic signaling
The phosphate-buffered system maintains solution pH within a stable range, supporting NAD⁺ structural integrity during experimental handling and storage.
Chemical Structure
Primary Identifying Compound:
Nicotinamide Adenine Dinucleotide (NAD⁺)
Chemical Name:
β-Nicotinamide adenine dinucleotide
Synonyms:
NAD⁺
Molecular Formula:
C₂₁H₂₇N₇O₁₄P₂
Molecular Weight:
663.43 g/mol
CAS Number:
53-84-9
Structural Description:
NAD⁺ is a dinucleotide composed of a nicotinamide moiety and an adenine nucleotide linked via a diphosphate bridge. This structure enables reversible redox cycling between NAD⁺ and NADH, which is fundamental to cellular energy metabolism and signaling. Structural identification and molecular data are available via PubChem.
Formulation Composition
Concentration:
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NAD⁺ (Nicotinamide Adenine Dinucleotide): 50 mg/mL
Buffer System:
Phosphate-buffered aqueous solution
Preservative:
Benzyl alcohol 1%
Physical and Chemical Properties
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Appearance: Clear, colorless to faintly yellow solution
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Solubility: Fully soluble aqueous formulation
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pH: Stabilized via phosphate buffer
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Stability: Improved stability compared to unbuffered NAD⁺ solutions when stored under cool, light-protected conditions
Research Focus Areas
NAD⁺ Phosphate Buffered Solution is commonly examined in laboratory research related to:
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Cellular redox and energy metabolism
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Mitochondrial function and bioenergetics
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NAD⁺-dependent enzyme activity (sirtuins, PARPs)
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Metabolic stress and aging-related research models
Research Use Disclaimer
NAD⁺ Phosphate Buffered Solution is intended for research use only.
Not for human consumption, therapeutic use, or diagnostic application. All handling and use must comply with applicable laws, regulations, and laboratory safety standards.
Summary
NAD⁺ Phosphate Buffered Solution is a stabilized aqueous research formulation containing 50 mg/mL nicotinamide adenine dinucleotide with verified chemical identifiers:
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CAS: 53-84-9
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Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
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Molecular Weight: 663.43 g/mol
Its buffered composition and defined concentration make it suitable for controlled laboratory studies focused on redox biology, mitochondrial metabolism, and NAD⁺-dependent signaling pathways.
| CAS Number | 53-84-9 |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 |
